External Services
Mouse Monoclonal Antibody Screening Technology Service
Business Introduction
Based on its own effective antibody screening technology platform, SGE BIOTECH is committed to providing customers with effective mouse monoclonal antibody screening services. At present, SGE BIOTECH has established a light-guide single-B antibody screening platform, a hybridoma single-cell sorting platform, and a mouse effective immunization platform. Technically, rapid and effective antibody screening can be achieved. Technically, rapid and effective antibody screening can be achieved.
✦ Antibody screening technology for light-guided single B
The light-guided single B cell antibody screening technology is a method based on high-throughput single cell screening, combined with microfluidic nanoscale micro-reaction chips, photoelectric positioning technology and computer system software control, it realizes the sorting and analysis of single B cells Integration of cultivation, exporting, accurate and non-destructive operation of thousands of single cells at the same time, and rapid and accurate screening of high-affinity and high-specificity antibodies. Compared with traditional antibody screening methods, light-guided single B cell antibody screening technology has the following significant advantages:
➢ High-throughput: It can screen thousands of individual B cells at the same time, greatly improving the screening efficiency.
➢ High sensitivity: It can detect low-frequency rare B cell clones and screen out high-affinity antibodies.
➢ High specificity: By screening a single B cell, it avoids clone mixing and non-specific reactions, and provides highly specific antibodies.
➢ Fast time: Antibody screening can be completed in a short period of time (within 1 week), speeding up the research process and drug development cycle.
✦ Single B sorting technology of hybridoma
SGE BIOTECH uses the self-developed antibody membrane anchoring technology to specifically anchor the antibody on the surface of the hybridoma cell that secretes the antibody, and then uses antigen fluorescent staining and flow cytometry sorting to sort the hybridoma that secretes the positive antibody. Sorting tumor cells into 96-well plates or 384-well plates to directly obtain antibody monoclonal cells. This operation avoids the cumbersome subcloning work of traditional hybridoma technology, greatly saves time, and at the same time increases the throughput of antibody screening by 2~ 3 orders of magnitude. 50-200 strains of strong positive antibodies can be obtained in a short period of time, and these antibodies can be effectively used for subsequent antibody activity evaluation, from which antibody molecules with target biological activity can be screened.
Technical Process
Service Content
1. Light guide single B antibody screening technology route
|
Content |
Description |
Cycle |
|
mouse immune |
Using the SGE BIOTECH immune platform technology, three immunizations of mice were completed, and the serum titer of mice was detected. |
5 weeks |
|
Single cell introduction and detection |
Take the mouse spleen, separate the plasma cells, import it into the light guide system chip, complete the online analysis. |
1 day |
|
Positive cell export |
B cells that secrete the target demand antibody are exported by photoelectric method. |
1 day |
|
antibody sequencing |
Single cell reverse transcription, nested PCR method to amplify the antibody sequence, complete the sequencing of the antibody light chain |
3-5 days |
2. Hybridoma single cell sorting technology route
|
Content |
Description |
Cycle |
|
mouse immune |
Using the SGE BIOTECH immune platform technology, three immunizations of mice were completed, and the serum titer of mice was detected. |
5 weeks |
|
Hybridoma fusion and sorting |
The fusion of hybridoma was completed by electrofusion instrument, and the single cell sorting of hybridoma was completed by flow cytometry. Single cell sorting of positive hybridomas into 96-well plates |
1 week |
|
hybridoma detection |
Take hybridoma supernatant to complete ELISA detection, FACS detection |
2 weeks |
|
antibody sequencing |
NGS antibody sequencing method to complete the sequencing of the antibody light chain, to obtain the antibody sequence |
1-2 weeks |
Service Process
Our Advantage
✦ Customized service: We provide flexible technical service solutions and carry out customized design according to the research needs and project characteristics of customers to ensure that the requirements of customers are met to the greatest extent.
✦ High-quality antibody delivery: Through the light-guided single B cell antibody screening technology and hybridoma sorting technology platform, we can provide high-affinity and high-specificity antibodies to provide strong support for customers' research and development work.
✦ Fast delivery: We have advanced experimental equipment and an experienced scientific research team, which can complete customer sample screening in a relatively short period of time and provide detailed experimental reports.
✦ Confidentiality and compliance: We attach great importance to and do a good job in the confidentiality of customers' research data and intellectual property rights.
If you are looking for a fast, accurate and reliable antibody screening method, our light-guided single B cell antibody screening technology service is your ideal choice. By choosing our services, you will obtain high-quality antibody screening results, which will bring new breakthroughs and success to your research and development projects. We look forward to working with you to advance scientific progress together!
Please contact our sales team, we will provide you with detailed solutions and quotations.
Ordering Information
Email: techsupport@sgebio.com
Phone: 400-185-1590