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Product Description（DESCRIPTION）
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species
Species
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Human
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molecular alias
Other name
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Accession
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Label
Tag
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Human IgG1 FC
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expression system
system
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Human embryonic kidney 293 cells, HEK293
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expression interval
Source
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/
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Quality Properties（SPECIFICATIONS）
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molecular weight
MW
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The protein migrates as 140kDa under non reducing (NR) condition (SDS-PAGE)
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activity
Activity
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Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
Optimal concentrations should be determined by each laboratory for each application.
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endotoxin
Endotoxin
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<1EU/UG
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Purity
Purity
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>95% as determined by SDS-PAGE.
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Preparation
Formulation
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Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
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save and use（PREPARATION AND STORAGE）
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Dissolution method
Reconstitution
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Reconstitute at 100 μg/mL in sterile PBS.
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Transportation
Shipping
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The product is shipped with Blue ice.
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Storage
Storage
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For long term storage, the product should be stored at lyophilized state at -20°C，avoid
repeated freezethaw cycles.
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Stability
Stability
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>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution
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Background Introduction（BACKGROUND）
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Wnt surrogate offers a novel solution to overcome the solubility challenge, which a major obstacle of working with Wnts. The Wnt surrogate is an engineered Fc fusion protein, which bridges the FZD and LRP5/6 receptors to activate Wnt signaling pathways with improved solubility and at much lower concentrations required compared to commercially available recombinant Wnt3a proteins or Wnt-conditioned media. The Wnt surrogate is composed of the LRP-binding domain of the extracellular Wnt modulator Dickkopf (DKK), linked to a designed ankyrin repeat protein (DARPin)—a selective, high affinity binder of Frizzled receptors. More specifically, this engineered Wnt mimetic interacts with FZD1, FZD2, FZD5, FZD7, and FZD8.
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