|
Product Description（DESCRIPTION）
|
|
species
Species
|
Human
|
|
molecular alias
Other name
|
/
|
|
Accession
|
UniProtKB# P56704
|
|
Label
Tag
|
Human IgG1 FC
|
|
expression system
system
|
Human embryonic kidney 293 cells, HEK293
|
|
expression interval
Source
|
Ser19Lys352, with FC tag at the C-terminal
|
|
Quality Properties（SPECIFICATIONS）
|
|
molecular weight
MW
|
Predicted Molecular Mass：63.3 kDa，The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
|
|
activity
Activity
|
/
|
|
endotoxin
Endotoxin
|
<1EU/UG
|
|
Purity
Purity
|
>75% as determined by SDS-PAGE.
|
|
Preparation
Formulation
|
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
|
|
save and use（PREPARATION AND STORAGE）
|
|
Dissolution method
Reconstitution
|
Reconstitute at 100 μg/mL in sterile PBS.
|
|
Transportation
Shipping
|
The product is shipped with Blue ice.
|
|
Storage
Storage
|
For long term storage, the product should be stored at lyophilized state at -20°C，avoid
repeated freezethaw cycles.
|
|
Stability
Stability
|
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution
|
|
Background Introduction（BACKGROUND）
|
|
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes . Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels
|
