Product Description (DESCRIPTION)
|
species
Species
|
Human
|
molecular alias
Other name
|
/
|
Accession
|
UniProt# Q2MKA7
|
Label
Tag
|
HIS
|
expression system
system
|
Human embryonic kidney 293 cells, HEK293
|
expression interval
Source
|
/
|
Mass Properties (SPECIFICATIONS)
|
molecular weight
MW
|
The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
|
activity
Activity
|
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Optimal concentrations should be determined by each laboratory for each application.
|
endotoxin
Endotoxin
|
<1EU/UG
|
Purity
Purity
|
>95% as determined by SDS-PAGE.
|
Preparation
Formulation
|
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 0.0%1 Tween 80 were added as protectant before lyophilization
|
Save and Use (PREPARATION AND STORAGE)
|
Dissolution method
Reconstitution
|
Reconstitute at 100 μg/mL in sterile PBS.
|
Transportation
Shipping
|
The product is shipped with Blue ice.
|
Storage
Storage
|
For long term storage, the product should be stored at lyophilized state at -20°C,avoid repeated freezethaw cycles.
|
Stability
Stability
|
>12 months, under -20°C conditions before reconstitution. >6 months, -80 °C under sterile conditions after reconstitution
|
Background Introduction (BACKGROUND)
|
R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat & Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R-Spondin-1 activates β-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK1 for binding to the WNT coreceptors, Kremen and LRP6, which reduces DKK1-mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells
|