Predicted Molecular Mass：63.3 kDa，The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
活性
Activity
/
内毒素
Endotoxin
<1EU/UG
纯度
Purity
>75% as determined by SDS-PAGE.
制剂
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
保存与使用（PREPARATION AND STORAGE）
溶解方法
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
运输
Shipping
The product is shipped with Blue ice.
存储
Storage
For long term storage, the product should be stored at lyophilized state at -20°C，avoid
repeated freezethaw cycles.
稳定性
Stability
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution
背景介绍（BACKGROUND）
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes . Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels
4%-20% SDS-PAGE Result Recombinant Wnt3a, 2ug reducing
