Neuregulin1 (NRG1-β1), Neu differentiation factor (rat), HRG, HRG1-β1, Breast cancer cell differentiation factor p45, ARIA (Acetylcholine, Receptor Inducing Activity), glial growth factor
Accession
Q02297
标签
Tag
HIS
表达系统
system
HEK293
表达区间
Source
Met1-Lys2468
质量属性（SPECIFICATIONS）
分子量
MW
25 kd
活性
Activity
Measured by its ability to bind HER3 by elisa method
内毒素
Endotoxin
<1.0 EU per 1 μg of the protein by the LAL method.
纯度
Purity
>95% as determined by SDS-PAGE.
制剂
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
保存与使用（PREPARATION AND STORAGE）
溶解方法
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
运输
Shipping
The product is shipped with blue ice.
存储
Storage
For long term storage, the product should be stored at lyophilized state at -20°C，avoid repeated freezethaw cycles.
稳定性
Stability
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution.
背景介绍（BACKGROUND）
Neuregulin/Heregulin is a family of structurally-related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation. Although HRG1-β1's biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells, which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF).
4%-20% SDS-PAGE Result Recombinant Human Heregulinβ-1, 2ug reducing
