human MSP/MST1 protein Gln19-Gly711，AAA59872（NCBI)
Species
Human
质量属性（SPECIFICATIONS）
SDSPAGE
The protein migrates 65 kDa ( alpha ‑chain) & 35 kDa( beta ‑chain), under reducing (R) condition (SDS-PAGE)
Purity
>80% as determined by SDS-PAGE.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
保存与使用（PREPARATION AND STORAGE）
Shipping
The product is shipped with dry ice.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C，avoid repeated freezethaw cycles.
Stability
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
背景介绍（BACKGROUND）
Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors . MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity . Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively . Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 - 62 kDa alpha and a 25-35 kDa beta chain . Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers . Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling . MSP induces macrophage and keratinocyte proliferation and osteoclast activation . It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM .
