Human embryonic kidney 293 cells, HEK293
Thr 32 - His 266, with a HIS tag at the C-terminal
Accession # NP_036374.1
Species
Human
质量属性（SPECIFICATIONS）
SDSPAGE
The protein migrates as 50 KDa under reducing (R) condition (SDS-PAGE) .
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.01 % Tween 80 were added as protectant before lyophilization
保存与使用（PREPARATION AND STORAGE）
Shipping
The product is shipped with dry ice.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS
Storage
For long term storage, the product should be stored at lyophilized state at -20°C，avoid repeated freezethaw cycles.
Stability
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution.
背景介绍（BACKGROUND）
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.
4%-20% SDS-PAGE Result
Recombinant DKK1, 2ug reducing
