- 产品描述
- 产品数据
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- 商品名称: Recombinant Human BAFF
- 商品编号: RP8064
- 物种: Human
- 表达系统: HEK293
- 标签: Human IgG1 FC
- 纯度: >95%
- 活性: 细胞活性
产品描述（DESCRIPTION）
Source
Human embryonic kidney 293 cells, HEK293
Ala134-Leu285, with a FC fusion tag at the N-terminal
Accession # AAH20674.1Species
Human
Predicted Molecular Mass
45 kDa
质量属性（SPECIFICATIONS）
SDSPAGE
The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.01% Tween 80 were added as protectant before lyophilization保存与使用（PREPARATION AND STORAGE）
Shipping
The product is shipped with Blue ice.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C，avoid repeated freezethaw cycles.
Stability
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution.背景介绍（BACKGROUND）
B-cell activating factor (BAFF) is also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.
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SDS-PAGE
4%-20% SDS-PAGE Result Recombinant BAFF, 2ug reducing